测序的深度的建议

Sequencing Coverage Requirements
https://www.illumina.com/science/technology/next-generation-sequencing/plan-experiments/coverage.html
Researchers typically determine the necessary NGS coverage level based on the method they're using, as well as other factors such as the reference genome size, gene expression levels, specific application of interest, published literature, and best practices from the scientific community. Examples of sequencing coverage recommendations for some common methods are listed here.

Sequencing Method	Recommended Coverage
Whole genome sequencing (WGS)	30× to 50× for human WGS (depending on application and statistical model)
Whole-exome sequencing	100×
RNA sequencing	Usually calculated in terms of numbers of millions of reads to be sampled. Detecting rarely expressed genes often requires an increase in the depth of coverage.
ChIP-Seq	100×