10X单细胞技术在肿瘤研究中的应用

Discover how single-cell RNA sequencing provides deeper insights into lung cancer biology

本文介绍了10X技术在肿瘤研究中的应用，从10X技术的介绍到具体的应用场景均有提及。数据挖掘作为一门学科，基本的思路和方法都是成体系得，所以数据挖掘的关键在于对问题的追问。

• 10X技术简介
• SCENIC
• 新的细胞类型
• 癌基因
• 亚群识别

这个图，值得注意，群定义。

再分群。

myc基因是较早发现的一组癌基因，包括C - myc，N -myc，L - myc ，分别定位于8号染色体，2号染色体和1号染色体。结构上由不编码蛋白质的第1外显子和编码蛋白质的第2、3外显子构成，与之同源的病毒癌基因存在于MC29及其它一些具有高度致癌性的猿逆转录病毒中。myc基因高水平表达时可转化啮齿类成纤维细胞。

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Presented By:
Bernard Thienpont - Assistant Professor, Faculty of Medicine, KU Leuven Speaker Biography: My doctoral research was performed at the Center for Human Genetics (KULeuven) under the guidance of Prof. K Devriendt, where we detected cryptic chromosomal aberrations as a novel cause for idiopathic congenital heart defects using arrayCGH. Our studies also identified a new genetic syndrome (16p13.3 duplication syndrome) and a new gene (TAB2) responsible for cardiac defects. Next, I moved to the Babraham Institute in Cambridge (UK), where I worked with Profs. HL Roderick and W Reik to study the epigenetic causes of cardiac hypertrophy, aiming to understand how pathologically hypertrophied hearts re-express their fetal gene programme.

Co-Presented By:
Annika Branting - Regional Marketing Manager, EMEA 10x Genomics Speaker Biography: Annika Branting works as a regional marketing manager in Europe, Middle East and Africa at 10x Genomics. In this role, she is responsible for identifying and driving marketing projects related to the single cell markets. Her prior roles at 10x Genomics span strategic marketing and support. Annika has a background in biotechnology and engineering with a MSc from the Royal Institute of Technology (KTH) in Stockholm. In her thesis work Annika spent a year as a visiting researcher at the Stanford Genome Technology Center in Palo Alto, California working on functionalized nano-pipettes as biosensors under professor Nader Pourmand.

Co-Presented By:
Pawel Zajac, Ph.D. - Segment Marketing Manager, Cell Biology, Illumina Speaker Biography: Pawel Zajac is the senior cell and molecular biology marketing manager in Europe, Middle East and Africa at Illumina. In this role, he is responsible for developing and executing the marketing plan for this segment. His prior roles at Illumina span several functions in sales and marketing. Pawel has a PhD from the Royal Institute of Technology (KTH) in Stockholm and worked as a PostDoc at the Karolinska Institute in Stockholm. His research focused on method development in single-cell RNA sequencing and multiplex amplification. Pawel is the author of 10 peer-reviewed articles in journals including Nature Methods and Genome Biology.

Webinar: Discover how single-cell RNA sequencing provides deeper insights into lung cancer biology Webinar Abstract: Single-cell genomics enables a deep dive into the mechanisms responsible for health and disease, in areas from cancer biology to neurobiology. This webinar will show how single-cell RNA sequencing enabled the creation of a lung tumor microenvironment catalog with more than 50,000 individual cells, offering novel insights into this complex ecosystem.

Presented by Dr. Bernard Thienpont from VIB in Leuven, Belgium. Learning Objectives: -Learn how single-cell sequencing with 10x Genomics and Illumina can enable new scientific discoveries -Learn how single-cell gene expression helped to expand our view of lung tumor microenvironment heterogeneity Annika will discuss: Transform your understanding of genomics, transcriptomics, and epigenomics—10x Technology and Applications Whether you want to dissect cell-type differences, investigate the adaptive immune system, or look at tumor heterogeneity on a single cell level, the Chromium System from 10x Genomics is the answer. In this brief presentation I will describe how to characterize and profile hundreds to millions of single cells, combine immune profiling with gene expression data for the same single cell and how to investigate cancer pathogenesis through single cell tumor sub-clones. Further more, the most impending product launches from 10x such as Single Cell ATAC solution and Single Cell Feature Barcode Solution will be briefly discussed. Learn how to enhance your biological discoveries with high-throughput single cell products from 10x Genomics.

Sponsored By: Illumina, 10x Genomics, Inc. Earn PACE Credits:
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