scale_fill_manual(values = c(“royalblue“, “red“))#scale_fill_gradientn(colors = colorRampPalette(c(“

library(ggplot2)
ggplot(subset_data@meta.data, 
       aes(x=Idents(subset_data),fill=DNM3OS_expr > 0)) + geom_bar(position = "fill")  +
  scale_fill_manual(values = c("royalblue", "red"))#scale_fill_gradientn(colors = colorRampPalette(c("royalblue", "firebrick3"))(12))#colorRampPalette(c("royalblue","firebrick3"))(2)