Frontiers | 在乳腺癌中，LAYN与免疫浸润相关并可作为指示预后的marker

LAYN Is a Prognostic Biomarker and Correlated With Immune Infiltrates in Gastric and Colon Cancers
这篇主要学习几个网站的使用

Introduction

LAYN是透明质酸（hyaluronan）的受体，在细胞粘附、运动、细胞扩散和迁移的调节中起重要作用。
Low levels of LAYN protein can reduce cell invasion and lymph node metastasis A549 lung cancer cells.

High LAYN expression was related to poor prognosis in CRC and NSCLC patients
LAYN is a crucial gene involved in liver cancer tumor-infiltrating lymphocytes.

Single- cell RNA sequencing of T cells confirmed that LAYN was up regulated in activated CD8+ T and Treg cells and represses CD8+ T cell functions in vitro.

Methods:

• Oncomine
  expression level of the LAYN gene in various types of cancers.
  threshold: P-value of 0.001, fold change of 1.5

• PrognoScan
  correlation between LAYN expression and survival in various types of cancers
  threshold:Cox P-value < 0.05

• Kaplan-Meier plotter
  主要用来做预后分析
  samples include 5,143 breast, 1,816 ovarian, 2,437 lung, and 1,065 gastric cancer samples on the HGU133 Plus 2.0 array with a mean follow-up of 69, 40, 49, and 33 months, respectively

• Tumor Immune Estimation Resource (TIMER)
  Systematic analysis of immune infiltrates across diverse cancer types
  TIMER applies a deconvolution previously published statistical method to infer the abundance of tumor-infiltrating immune cells (TIICs) from gene expression profiles. The TIMER database includes 10,897 samples across 32 cancer types from The Cancer Genome Atlas (TCGA) to estimate the abundance of immune infiltrates.

• Gene Expression Profiling Interactive Analysis(GEPIA)
  GEPIA was used to generate survival curves, including OS and DFS, based on gene expression with the log-rank test and the Mantel- Cox test in 33 different types of cancer. The Spearman method was used to determine the correlation coefficient.

• Statiistical Analysis
  strength of the correlation was determined using the following guide for the absolute value: 0.00–0.19 “very weak,” 0.20–0.39 “weak,” 0.40–0.59 “moderate,” 0.60–0.79 “strong,” 0.80–1.0 “very strong.” P-values <0.05 were considered statistically significant

Results

• LAYN expression was higher in breast, colorectal, gastric,kidney, pancreatic cancers, and lymphoma tumors compared to the normal tissues. In addition, lower expression kidney, pancreatic cancers, and lymphoma tumors compared to the normal tissues

LAYN expression levels in different types of human cancers

• Prognostic Potential of LAYN in Cancers(PrognoScan&Kaplan-Meier plotter)

  
  
  Kaplan-Meier survival curves comparing the high and low expression of LAYN in different types of cancer in the PrognoScan and Kaplan-Meier plotter databases

*LAYN Expression Is Correlated With Immune Infiltration Level in Colon and Gastric Cancers(TIMER&GEPIA）
不仅可以做基因与免疫浸润的关系，也可以做基因与基因的correlation

Correlation of LAYN expression with immune infiltration level in COAD, LUSC, STAD

LAYN expression correlated with markers of immune cells