Week 6 L11-12

http://mooc.guokr.com/note/17258/

11.1 错配修复Introduction to DNA MIsmatch Repair错配修复（MMR）

从此开始讲DNA修复。dna错配有两种类型：复制错误和DNA损伤

11.2 错配识别

错配识别由一种称为MutS的蛋白质介导

11.3MutS Recognition

MutS把错配的位点识别出来

11.4 Methyl-Directed Nascent Strand Detection

通过甲基化修饰来识别新生链，大肠杆菌中。因为新生链是未被甲基化的链。

甲基化位点为了保护大肠杆菌的DNA使其不在GATC位点被切

11.5MutH Recognition

MutH酶执行辨认工作。是一个在GATC位点切割未甲基化链的内切酶。从而会切割错配的新生链。

11.6 缺口介导的新生链检测Nick-Directed Nascent Strand Detection

利用了新DNA有缺口的特点。检测滑行夹是否存在

11.7MutL Regulation in Other Organisms

11.8 Choosing Which Strand to Repair

11.9Methyl-Directed Strand Repair

11.10Nick-Directed Strand Repair

11.10bAdditional Information

12.1Methyl-Directed MMR Assays

12.2Interpreting Methyl-Directed MMR Assay Results
12.3 In Vivo Reversion Assays
12.4In Vitro MMR Assay
12.5In Vitro MMR Incision Assay and Methylation Detection