NCBI Blast Tutorial

Introduction

NCBI

National Center for Biotechnology Information 美国国家生物技术信息中心

Database	Intro
GenBank	DNA sequences
PubMed	biomedical literature
Epigenomics	Other

The NCBI houses a series of databases relevant to biotechnology and biomedicine and an important resource for bioinformatics tools and services. Major databases include GenBank for DNA sequences and PubMed, a bibliographic database for the biomedical literature. Other databases include the NCBI Epigenomics database. All these databases are available online through the Entrez search engine.

Blast

BLAST finds regions of similarity between biological sequences.
寻找生物序列之间的相似性
The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance.

Quick Start

NCBI Home

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NCBI - BLAST
https://blast.ncbi.nlm.nih.gov/Blast.cgi

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Tools	Function
Nucleotide BLAST	根据DNA检索DNA相似序列
blastx	根据DNA检索转录出来的蛋白质序列
tblastn	根据蛋白质序列检索对应的DNA序列
Protein BLAST	根据蛋白质序列检索蛋白质序列

这里我们根据DNA序列来检索， 所以选择Nucleotide BLAST

物种 人类

待搜索的DNA序列

TAGTGAGTTATCGAACTTGAGGGCAT

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获取结果

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Reference

https://www.youtube.com/watch?v=hTTvq8KJthA&spfreload=5