【糖尿病肌病.2017】α-硫辛酸对糖尿病肌病的影响

文献DOI：10.1007/s40618-017-0720-0

文献PMID：28660607

文献原文链接：http://doi.org.sci-hub.tw/10.1007/s40618-017-0720-0

The effects of alpha‑lipoic acid on diabetic myopathy

α-硫辛酸对糖尿病肌病的影响

【Abstract】

Purpose： Increased oxidative stress and impaired antioxidant defense are important mechanisms in the pathogenesis of diabetic myopathy. Since diabetes mellitus type 1 decreases muscle regeneration capacity the present study was designed to determine the infuence of alpha-lipoic acid (ALA), a potent biological antioxidant, on the process of regeneration of diabetic rat skeletal muscles.

Methods： 40 Wistar rats were divided into three groups: control (n = 8), untreated diabetic group (n = 16) and ALA treated diabetic group (n = 16). The regeneration process was provoked in streptozotocin-induced diabetic rats in both slow (m.soleus, SOL) and fast (m.extensor digitorum longus, EDL) skeletal muscles by intramuscular injection of myotoxin bupivacaine. At intervals of 10 days and 4 weeks, muscle histochemical and morphometrical analysis (fber cross areas and fber type distribution) was performed.

Results： Changes induced by diabetes are evident in redistribution of muscle fbers and in signifcant level of atrophy. After 4 weeks of diabetes, glycolytic muscle fbers are dominant in both slow and fast muscles. Muscle atrophy is present in all fber types except in type I of slow skeletal muscle. Treatment with ALA reduce changes in the morphological properties caused by diabetes mellitus type 1 in slow and fast rat skeletal muscles during the process of regeneration.

Conclusion：Treatment with lipoic acid during 4 weeks has shown effects on the redistribution of muscle fbers, and can prevent atrophy in slow and fast diabetic muscle.

Keywords： Skeletal muscle · Regeneration · Diabetes mellitus type 1 · Alpha-lipoic acid

摘要翻译：

目的：增加氧化应激和抗氧化防御受损是糖尿病肌病发病机制的重要机制。由于1型糖尿病降低了肌肉再生能力，本研究旨在确定α-硫辛酸（ALA）（一种有效的生物抗氧化剂）对糖尿病大鼠骨骼肌再生过程的影响。

方法：将40只Wistar大鼠分为3组：对照组（n = 8），未治疗的糖尿病组（n = 16）和ALA治疗的糖尿病组（n = 16）。通过肌内注射肌肉毒素布比卡因，在慢性（m.soleus，SOL）和快速（m.extensor digitorum longus，EDL）骨骼肌中的链脲佐菌素诱导的糖尿病大鼠中引起再生过程。以10天和4周的间隔，进行肌肉组织化学和形态计量学分析（纤维交叉区域和纤维类型分布）。

结果：糖尿病引起的变化在肌肉纤维重新分布和显著萎缩水平中是明显的。糖尿病4周后，慢速和快速肌肉的肌纤维都是以糖酵解占主导地位。除了缓慢骨骼肌的I型外，所有肌肉类型都存在肌肉萎缩。 ALA治疗减少了在慢速和快速大鼠骨骼肌中再生过程中由1型糖尿病引起的形态学特性的变化。

结论：4周内用硫辛酸治疗对肌肉纤维的再分布有一定的作用，可以预防慢性和快速糖尿病肌肉的萎缩。

关键词：骨骼肌·再生·糖尿病1型·α-硫辛酸