WEEK 6 Live-cell Imaging for Single Cell Protein Dynamics

Lecture 1 - Fluorescence Microscopy荧光显微法

显微镜的结构和原理

Lecture 2 - Types of Imaging

不同种类的显微镜

Lecture 3 - Visualizing Molecules in Living Cells: Fluorescent Tools

Small Molecule Dyes

Hoechst给DNA和核染色
Mitotracker给线粒体染色

Biosensors

例如将荧光蛋白跟目标蛋白结合
FRET可以看蛋白蛋白相互作用

Lecture 4 - Quantification

Lecture 5 - Lab-Based Video

使用FRET定量、

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