2016-Nat Commun-A systems study reveals concurrent activation of AMPK and mTOR by amino acids

以12年发表的两篇文章为基础。
先找到最佳的模型，然后用实验
We find that aa acutely activate AMPK concurrently with mTOR. We show that AMPK under aa sufficiency acts to sustain autophagy. This may be required to maintain protein homoeostasis自稳态 and deliver metabolite intermediates代谢物中间体 for biosynthetic processes生物合成过程.

1. Cells were lysed at different time points post stimulation from 1 to 120 min做了一个时间梯度
2. FLAG-ULK1-WT or FLAG-ULK1-S758EAntibody
3. Quantitative SILAC-based phosphoproteomics （ LTQ Orbitrap、C18、MaxQuant、UniProt mouse protein sequence database、at least two existing、t-test P-value0.05
4. 注意其中的MODELwe used computational modelling as a means to broaden the scope and seek other aa inputs to the mTOR network模拟多个AA的输入过程。找到最优的拟合模型
   【待解决的问题】
5. 需要了解怎样构建模型，可以学习Mount Sinai Medical Center的Ravi Iyengar教授讲的系统生物学的教程。