测序深度和覆盖度(Sequencing depth and coverage)

总是跑数据,却对数据一无所知,这说不过去吧。

 

看几篇文章吧

Sequencing depth and coverage: key considerations in genomic analyses(只讲二代)

Assembly of large genomes using second-generation sequencing(参考文献)

Identification of optimum sequencing depth especially for de novo genome assembly of small genomes using next generation sequencing data(二代最佳测序深度:50X)

Large Genome Assembly with PacBio Long Reads(已更新)(纯三代最少50X,二三代联合:任意深度,策略不同)

 

回答几个问题:

不同的测序(denovo组装、重测序、转录组、外显子、表观),各对数据的深度和覆盖度有什么要求?

二代和三代在测序深度和覆盖度上的要求有什么不同?

 

four major study designs:

  1. de novo genome sequencing
  2. genome resequencing
  3. transcriptome sequencing
  4. genomic location analyses (for example, chromatin immunoprecipitation followed by sequencing (ChIP–seq) and chromosome conformation capture (3C))

 

参考资料:

Question: de novo sequence assembly with extremely high coverage

 

待续~

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