到底什么是Lin single lung IMs (Lin−CD45+MerTK+CD64+SiglecF−CD11b+) were collected by FACS into 96-well pl

经常有老师看文献时看到参考指标时会时不时的蹦出来一个lin-,然后查抗体时却发现怎么查怎么都查不到这个lin的抗体,究竟是怎么一回事呢?

这期罗工秘笈就来和大家聊聊这个熟悉而又陌生的指标:Lin。

那么到底什么是lin呢?

lin的全称又叫Lineage,或者Lineage cocktail,这个指标在流式上并不是指的某一个指标,而是指的某一类指标的集合体。

比如在做human DC细胞的鉴定时,Lin就是CD3/CD19/CD20/CD14等指标的集合指标,把这些抗体混合在一起,就叫做DC细胞的lin-指标。

到底什么是Lin single lung IMs (Lin−CD45+MerTK+CD64+SiglecF−CD11b+) were collected by FACS into 96-well pl_第1张图片

Historical 4-color staining method: cluster resolution and phenotypic characteristics of mDC and pDC subsets. DC subsets were stained with lineage (lin) mAb cocktail (anti-CD3,−14,−19,−20) and with anti-CD11c and anti-CD123 (IL-3R α). mDC are lin- CD11c+ HLA-DR+ cells; pDC are lin-, CD123bright HLA-DR+.

Mario G. Solari, et al. Transplantation. ;85(11):1513-1522.

那么为什么要进行lin-的鉴定的?简单的来说,就是为了排除法。

还是以上面的DC细胞为例&

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