library(ggplot2)
library(ggpubr)
library(qtl)
f2<- read.cross(format="csvr",dir="",
file="F2P05M30-binr1m5k-Raw.csv",genotypes=c("A","H","B"),
alleles=c("A","B"),na.strings=c("N"))
##修改R::plotMap的参数，调试染色体的宽度和间距
plotMap<-function (x, map2, chr, horizontal = FALSE, shift = TRUE, show.marker.names = FALSE,
alternate.chrid = FALSE, ...)
{
dots <- list(...)
if ("main" %in% names(dots)) {
themain <- dots$main
usemaindefault <- FALSE
}
else usemaindefault <- TRUE
if ("xlim" %in% names(dots)) {
xlim <- dots$xlim
usexlimdefault <- FALSE
}
else usexlimdefault <- TRUE
if ("ylim" %in% names(dots)) {
ylim <- dots$ylim
useylimdefault <- FALSE
}
else useylimdefault <- TRUE
if ("xlab" %in% names(dots))
xlab <- dots$xlab
else {
if (horizontal)
xlab <- "Location (cM)"
else xlab <- "Chromosome"
}
if ("ylab" %in% names(dots))
ylab <- dots$ylab
else {
if (horizontal)
ylab <- "Chromosome"
else ylab <- "Location (cM)"
}
map <- x
if (inherits(map, "cross"))
map <- pull.map(map)
if (!missing(map2) && inherits(map2, "cross"))
map2 <- pull.map(map2)
if (!inherits(map, "map") || (!missing(map2) && !inherits(map2,
"map")))
warning("Input should have class \"cross\" or \"map\".")
if (!missing(map2) && is.matrix(map[[1]]) != is.matrix(map2[[1]]))
stop("Maps must be both sex-specific or neither sex-specific.")
if (!missing(chr)) {
map <- map[matchchr(chr, names(map))]
if (!missing(map2))
map2 <- map2[matchchr(chr, names(map2))]
}
sex.sp <- FALSE
if (is.matrix(map[[1]])) {
one.map <- FALSE
sex.sp <- TRUE
if (!missing(map2)) {
if (is.logical(map2)) {
horizontal <- map2
map2 <- lapply(map, function(a) a[2, ])
map <- lapply(map, function(a) a[1, ])
}
else {
Map1 <- lapply(map, function(a) a[1, , drop = TRUE])
Map2 <- lapply(map, function(a) a[2, , drop = TRUE])
Map3 <- lapply(map2, function(a) a[1, , drop = TRUE])
Map4 <- lapply(map2, function(a) a[2, , drop = TRUE])
old.mfrow <- par("mfrow")
on.exit(par(mfrow = old.mfrow))
par(mfrow = c(2, 1))
class(Map1) <- class(Map2) <- class(Map3) <- class(Map4) <- "map"
plotMap(Map1, Map3, horizontal = horizontal,
shift = shift, show.marker.names = show.marker.names,
alternate.chrid = alternate.chrid)
plotMap(Map2, Map4, horizontal = horizontal,
shift = shift, show.marker.names = show.marker.names,
alternate.chrid = alternate.chrid)
return(invisible(NULL))
}
}
else {
map2 <- lapply(map, function(a) a[2, ])
map <- lapply(map, function(a) a[1, ])
}
}
else {
if (!missing(map2))
one.map <- FALSE
else one.map <- TRUE
}
if (one.map) {
n.chr <- length(map)
if (!show.marker.names) {
chrpos <- 1:n.chr
thelim <- range(chrpos) + c(-0.5, 0.5)
}
else {
chrpos <- seq(1, n.chr * 2, by = 2) ##2->10
thelim <- range(chrpos) + c(-0.35, 2.35)
}
if (shift)
map <- lapply(map, function(a) a - a[1])
maxlen <- max(unlist(lapply(map, max)))
if (horizontal) {
old.xpd <- par("xpd")
old.las <- par("las")
par(xpd = TRUE, las = 1)
on.exit(par(xpd = old.xpd, las = old.las))
if (usexlimdefault)
xlim <- c(0, maxlen)
if (useylimdefault)
ylim <- rev(thelim)
plot(0, 0, type = "n", xlim = xlim, ylim = ylim,
yaxs = "i", xlab = xlab, ylab = ylab, yaxt = "n")
a <- par("usr")
for (i in 1:n.chr) {
segments(min(map[[i]]), chrpos[i], max(map[[i]]),
chrpos[i])
### not this line
### segments(map[[i]], chrpos[i] - 0.25, map[[i]],
### not this line
### chrpos[i] + 0.25)
segments(map[[i]], chrpos[i] - 0.25, map[[i]],
chrpos[i] + 0.25)
if (show.marker.names)
### not this line
### text(map[[i]], chrpos[i] + 0.35, names(map[[i]]),
### not this line
### srt = 90, adj = c(1, 0.5))
text(map[[i]], chrpos[i] + 0.35, names(map[[i]]),
srt = 90, adj = c(0.1, 0.5))
}
if (!alternate.chrid || length(chrpos) < 2) {
for (i in seq(along = chrpos)) axis(side = 2,
at = chrpos[i], labels = names(map)[i])
}
else {
odd <- seq(1, length(chrpos), by = 2) ##2->10
even <- seq(2, length(chrpos), by = 2) ##2->10
for (i in odd) {
axis(side = 2, at = chrpos[i], labels = "")
axis(side = 2, at = chrpos[i], labels = names(map)[i],
### not this line
### line = -0.4, tick = FALSE)
line = -0.4, tick = FALSE)
}
for (i in even) {
axis(side = 2, at = chrpos[i], labels = "")
axis(side = 2, at = chrpos[i], labels = names(map)[i],
### not this line
### line = +0.4, tick = FALSE)
line = +0.4, tick = FALSE)
}
}
}
else {
old.xpd <- par("xpd")
old.las <- par("las")
par(xpd = TRUE, las = 1)
on.exit(par(xpd = old.xpd, las = old.las))
if (usexlimdefault)
xlim <- thelim
if (useylimdefault)
ylim <- c(maxlen, 0)
plot(0, 0, type = "n", ylim = ylim, xlim = xlim,
xaxs = "i", xlab = xlab, ylab = ylab, xaxt = "n")
a <- par("usr")
for (i in 1:n.chr) {
segments(chrpos[i], min(map[[i]]), chrpos[i],
max(map[[i]]))
################ 控制标记线长度 ################### 下方是原数值
###just this line segments(chrpos[i] - 0.25, map[[i]], chrpos[i] +
###just this line 0.25, map[[i]])
### if (show.marker.names)
### text(chrpos[i] + 0.35, map[[i]], names(map[[i]]),
### adj = c(0, 0.5))
################ 控制标记线长度 ################### 下方是修改数值
segments(chrpos[i] - 0.048, map[[i]], chrpos[i] +
0.048, map[[i]])
if (show.marker.names)
text(chrpos[i] + 0.35, map[[i]], names(map[[i]]),
adj = c(0, 0.5))
################ 控制标记线长度 ###################
}
if (!alternate.chrid || length(chrpos) < 2) {
for (i in seq(along = chrpos)) axis(side = 1,
at = chrpos[i], labels = names(map)[i])
}
else {
odd <- seq(1, length(chrpos), by = 2) ##2->10
even <- seq(2, length(chrpos), by = 2) ##2->10
for (i in odd) {
axis(side = 1, at = chrpos[i], labels = "")
axis(side = 1, at = chrpos[i], labels = names(map)[i],
### not this line
### line = -0.4, tick = FALSE)
line = -0.4, tick = FALSE)
}
for (i in even) {
axis(side = 1, at = chrpos[i], labels = "")
axis(side = 1, at = chrpos[i], labels = names(map)[i],
### not this line
### line = +0.4, tick = FALSE)
line = +0.4, tick = FALSE)
}
}
}
if (usemaindefault)
title(main = "Genetic map")
else if (themain != "")
title(main = themain)
}
else {
map1 <- map
if (is.matrix(map2[[1]]))
stop("Second map appears to be a sex-specific map.")
if (length(map1) != length(map2))
stop("Maps have different numbers of chromosomes.")
if (any(names(map1) != names(map2))) {
cat("Map1: ", names(map1), "\n")
cat("Map2: ", names(map2), "\n")
stop("Maps have different chromosome names.")
}
if (shift) {
map1 <- lapply(map1, function(a) a - a[1])
map2 <- lapply(map2, function(a) a - a[1])
}
n.mar1 <- sapply(map1, length)
n.mar2 <- sapply(map2, length)
markernames1 <- lapply(map1, names)
markernames2 <- lapply(map2, names)
if (any(n.mar1 != n.mar2)) {
if (show.marker.names) {
warning("Can't show marker names because of different numbers of markers.")
show.marker.names <- FALSE
}
}
else if (any(unlist(markernames1) != unlist(markernames2))) {
if (show.marker.names) {
warning("Can't show marker names because markers in different orders.")
show.marker.names <- FALSE
}
}
n.chr <- length(map1)
maxloc <- max(c(unlist(lapply(map1, max)), unlist(lapply(map2,
max))))
if (!show.marker.names) {
chrpos <- 1:n.chr
thelim <- range(chrpos) + c(-0.5, 0.5)
}
else {
chrpos <- seq(1, n.chr * 2, by = 2) ##2->10
thelim <- range(chrpos) + c(-0.4, 2.4)
}
if (!horizontal) {
old.xpd <- par("xpd")
old.las <- par("las")
par(xpd = TRUE, las = 1)
on.exit(par(xpd = old.xpd, las = old.las))
if (usexlimdefault)
xlim <- thelim
if (useylimdefault)
ylim <- c(maxloc, 0)
plot(0, 0, type = "n", ylim = ylim, xlim = xlim,
xaxs = "i", xlab = xlab, ylab = ylab, xaxt = "n")
a <- par("usr")
for (i in 1:n.chr) {
if (max(map2[[i]]) < max(map1[[i]]))
map2[[i]] <- map2[[i]] + (max(map1[[i]]) -
max(map2[[i]]))/2
else map1[[i]] <- map1[[i]] + (max(map2[[i]]) -
max(map1[[i]]))/2
segments(chrpos[i] - 0.3, min(map1[[i]]), chrpos[i] -
0.3, max(map1[[i]]))
segments(chrpos[i] + 0.3, min(map2[[i]]), chrpos[i] +
0.3, max(map2[[i]]))
wh <- match(markernames1[[i]], markernames2[[i]])
for (j in which(!is.na(wh))) segments(chrpos[i] -
0.3, map1[[i]][j], chrpos[i] + 0.3, map2[[i]][wh[j]])
if (any(is.na(wh)))
segments(chrpos[i] - 0.4, map1[[i]][is.na(wh)],
chrpos[i] - 0.2, map1[[i]][is.na(wh)])
wh <- match(markernames2[[i]], markernames1[[i]])
if (any(is.na(wh)))
segments(chrpos[i] + 0.4, map2[[i]][is.na(wh)],
chrpos[i] + 0.2, map2[[i]][is.na(wh)])
if (show.marker.names)
text(chrpos[i] + 0.35, map2[[i]], names(map2[[i]]),
adj = c(0, 0.5))
}
if (!alternate.chrid || length(chrpos) < 2) {
for (i in seq(along = chrpos)) axis(side = 1,
at = chrpos[i], labels = names(map1)[i])
}
else {
odd <- seq(1, length(chrpos), by = 2) ##2->10
even <- seq(2, length(chrpos), by = 2) ##2->10
for (i in odd) {
axis(side = 1, at = chrpos[i], labels = "")
axis(side = 1, at = chrpos[i], labels = names(map1)[i],
### not this line
### line = -0.4, tick = FALSE)
line = -0.4, tick = FALSE)
}
for (i in even) {
axis(side = 1, at = chrpos[i], labels = "")
axis(side = 1, at = chrpos[i], labels = names(map1)[i],
### not this line
### line = +0.4, tick = FALSE)
line = +0.4, tick = FALSE)
}
}
}
else {
old.xpd <- par("xpd")
old.las <- par("las")
par(xpd = TRUE, las = 1)
on.exit(par(xpd = old.xpd, las = old.las))
if (usexlimdefault)
xlim <- c(0, maxloc)
if (useylimdefault)
ylim <- rev(thelim)
plot(0, 0, type = "n", xlim = xlim, ylim = ylim,
xlab = xlab, ylab = ylab, yaxt = "n", yaxs = "i")
a <- par("usr")
for (i in 1:n.chr) {
if (max(map2[[i]]) < max(map1[[i]]))
map2[[i]] <- map2[[i]] + (max(map1[[i]]) -
max(map2[[i]]))/2
else map1[[i]] <- map1[[i]] + (max(map2[[i]]) -
max(map1[[i]]))/2
segments(min(map1[[i]]), chrpos[i] - 0.3, max(map1[[i]]),
chrpos[[i]] - 0.3)
segments(min(map2[[i]]), chrpos[i] + 0.3, max(map2[[i]]),
chrpos[[i]] + 0.3)
wh <- match(markernames1[[i]], markernames2[[i]])
for (j in which(!is.na(wh))) segments(map1[[i]][j],
chrpos[i] - 0.3, map2[[i]][wh[j]], chrpos[i] +
0.3)
if (any(is.na(wh)))
segments(map1[[i]][is.na(wh)], chrpos[i] -
0.4, map1[[i]][is.na(wh)], chrpos[i] - 0.2)
wh <- match(markernames2[[i]], markernames1[[i]])
if (any(is.na(wh)))
segments(map2[[i]][is.na(wh)], chrpos[i] +
0.4, map2[[i]][is.na(wh)], chrpos[i] + 0.2)
if (show.marker.names)
text(map2[[i]], chrpos[i] + 0.35, names(map2[[i]]),
srt = 90, adj = c(1, 0.5))
}
if (!alternate.chrid || length(chrpos) < 2) {
for (i in seq(along = chrpos)) axis(side = 2,
at = chrpos[i], labels = names(map1)[i])
}
else {
odd <- seq(1, length(chrpos), by = 2) ##2->10
even <- seq(2, length(chrpos), by = 2) ##2->10
for (i in odd) {
axis(side = 2, at = chrpos[i], labels = "")
axis(side = 2, at = chrpos[i], labels = names(map1)[i],
### not this line
### line = -0.4, tick = FALSE)
line = -0.4, tick = FALSE)
}
for (i in even) {
axis(side = 2, at = chrpos[i], labels = "")
axis(side = 2, at = chrpos[i], labels = names(map1)[i],
### not this line
### line = +0.4, tick = FALSE)
line = +0.4, tick = FALSE)
}
}
}
if (usemaindefault) {
if (!sex.sp)
title(main = "Comparison of genetic maps")
else title(main = "Genetic map")
}
else if (themain != "")
title(main = themain)
}
invisible()
}
#
par(mfrow=c(2,1))