2009-Proteomics-Global analysis of cellular protein translation by pulsed SILAC

Here, we developed pulsed stable isotope labeling by amino acids in cell culture (pSILAC) with two heavy isotope labels to directly quantify protein translation on a proteome-wide scale. We applied the method to cellular iron homeostasis as a model system and demonstrate that it can confidently identify proteins that are translationally regulated by iron availability.

1. 研究计划5）中使用的pulse silac labeling方法。之所以使用这种方法是因为Building the ratio for each protein between the unlabelled and labelled moiety will allow determining the extent to which each protein of the proteome has been newly synthesized upon stress.
2. 研究计划5）为选修，可跳过。