2016-JPR-Translational targeted proteomics profiling of mitochondrial energy metabolic pathways in mouse and human samples

1. 靶向蛋白质组学作为一种可以替代ELISA or immunoblotting的方法。
2. 摘要
   We developed targeted LC-MS/MS assays in the Selected Reaction Monitoring (SRM) mode to quantify over 50 mitochondrial proteins in a single run. The targeted proteins cover the tricarboxylic acid cycle, fatty acid β-oxidation, oxidative phosphorylation and the detoxification of reactive oxygen species.
3. 将这种absolute quantitative targeted proteomic data方法鉴定的结果构建的模型与根据semi-quantitative immonoblot data构建的模型相比较。