20221109 今日文献翻译

p35 is an unstable protein with a half-life of 30 – 60 min, and it is degraded by proteasomes after ubiquitination (12, 13, 17). 

P35是一个不稳定的蛋白质，半衰期约为30-60min，会被泛素化修饰后由蛋白酶体降解

Because the p35 protein amount is a critical determinant of Cdk5 activity, elucidating the p35 degradation mechanism is central for understanding Cdk5 functions. 

因为p35蛋白是CDK5活性的主要决定因素，阐明p35的降解机制对于CDK5的功能十分重要

To this end we searched for the polyubiquitination site(s) in p35, but unexpectedly, we found that the degradation of p35 can occur without ubiquitination. 

我们在最后找到了p35的多聚泛素化位点，但出乎意料的是，我们发现p35的降解也可以在没有泛素化的情况下发生

We also showed that ubiquitin-independent degradation was mediated by an α-helical degron-like sequence in the C-terminal region of p35. 

我们还发现非依赖泛素降解途径是由类似于p35C末端结构域的α螺旋结构介导的

Thus, p35 is subjected to two different degradation mechanisms: ubiquitin-dependent and ubiquitin-independent.

因此，p35可以有两种不同的降解机制：泛素化和非泛素化