blastall报错之一

[blastall] WARNING: Contig41035: Could not calculate ungapped Karlin-Altschul parameters due to an invalid query sequence or its translation. Please verify the query sequence(s) and/or filtering options

baidu或者bing：
相关回答比较少

主要有两个回答：

1.fa序列存在N
2.序列太短

解决方法：加上 -F F
blastall -F F
-F 默认为T

参考：
https://www.biostars.org/p/167725/
http://blog.sina.com.cn/s/blog_8a216d8f0102v373.html
https://www.newsmth.net/bbsanc.php?path=%2Fgroups%2Fsci.faq%2FBioinformatics%2Fsoftware%2FM.1253860507.P0