NovaSeq™ 6000 System Quality Scores and RTA3 Software - Illumina
NovaSeq测序仪更改了碱基质量打分的方法,详见下面文章https://www.illumina.com/content/dam/illumina-marketing/documents/products/appnotes/novaseq-hiseq-q30-app-note-770-2017-010.pdf所以,下机fastq文件里碱基质量长下面这样,只有F,,,:三种符号,一般碱基质量都是用